Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes

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چکیده

Abstract The aim of this study was to investigate the presence virulence and antimicrobial resistance genes clonal profile Enterobacteriaceae isolated from calves, cows, feeding buckets milk bucket. A a total 31 isolates calves (6), bucket (6) cows’ rectum (13) were used. LT, STa, STb, STx1, STx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH uge as well genes: AmpC MOX, FOX, MIR, ACT, DHA, ACC, CTX-M-1, CTX-M2, TEM KPC MCR-1 SH evaluated by PCR. LT toxin gene detected in five (16.1%) mrkD three (9.0%). CTX-M-1 13 (41.9%), CTX-M-2 ACC-M four (12.9%). Most obtained demonstrated, cephalothin (87.5%), ampicillin, (87.5%) streptomycin (84.3%) with multidrug observed all isolates. Isolates cow’s rectum, bucket, calf shared same pulsotypes. These findings may suggest that enterobacteria carring persist environment became reservoir these genes.

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ژورنال

عنوان ژورنال: Food Science and Technology

سال: 2021

ISSN: ['2331-513X', '2331-5156']

DOI: https://doi.org/10.1590/fst.37819